UNIT 18.9 In Vitro Model for Modulation of Helper T Cell Differentiation and Activation
Published Online: 1 JUN 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Heo, Y. 2005. In Vitro Model for Modulation of Helper T Cell Differentiation and Activation. Current Protocols in Toxicology. 24:18.9.1–19.9.10.
- Published Online: 1 JUN 2005
- Published Print: MAY 2005
Influence of immunotoxicants on helper T cell reactivities can be investigated by assessing acquisition of IL-4- or IFN-γ-producing ability in vitro with antigen-primed naive or precursor helper T cells from the spleens of DO11.10 ovalbumin-specific transgenic mice. The in vitro differentiation model is believed to be close to the in vivo environmental conditions in which differentiation occurs. The effect of immunotoxicants on helper T cell activity can also be evaluated by antigen-specific activation of cloned type-1 or type-2 helper T cells in vitro, since the two subsets of helper T cells can be distinguished by patterns of cytokine secretion. This in vitro model will help investigators to examine the ability of toxicants to modulate helper T cell–mediated cellular or humoral immunity.
- OVA-tg mice;
- helper T cell;
- helper T cell clone;
- in vitro;
- rabbit IgG