Unit

UNIT 18.9 In Vitro Model for Modulation of Helper T Cell Differentiation and Activation

  1. Yong Heo

Published Online: 1 JUN 2005

DOI: 10.1002/0471140856.tx1809s24

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Heo, Y. 2005. In Vitro Model for Modulation of Helper T Cell Differentiation and Activation. Current Protocols in Toxicology. 24:18.9:18.9.1–19.9.10.

Author Information

  1. Catholic University of Daegu, Kyongbuk Province Republic of Korea

Publication History

  1. Published Online: 1 JUN 2005
  2. Published Print: MAY 2005

This is not the most recent version of the article. View current version (1 FEB 2016)

Abstract

Influence of immunotoxicants on helper T cell reactivities can be investigated by assessing acquisition of IL-4- or IFN-γ-producing ability in vitro with antigen-primed naive or precursor helper T cells from the spleens of DO11.10 ovalbumin-specific transgenic mice. The in vitro differentiation model is believed to be close to the in vivo environmental conditions in which differentiation occurs. The effect of immunotoxicants on helper T cell activity can also be evaluated by antigen-specific activation of cloned type-1 or type-2 helper T cells in vitro, since the two subsets of helper T cells can be distinguished by patterns of cytokine secretion. This in vitro model will help investigators to examine the ability of toxicants to modulate helper T cell–mediated cellular or humoral immunity.

Keywords:

  • OVA-tg mice;
  • helper T cell;
  • differentiation;
  • helper T cell clone;
  • activation;
  • in vitro;
  • rabbit IgG