UNIT 18.10 Host Resistance Model to an Intracellular Pathogen

  1. Ling Cao

Published Online: 1 MAR 2006

DOI: 10.1002/0471140856.tx1810s27

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Cao, L. 2006. Host Resistance Model to an Intracellular Pathogen. Current Protocols in Toxicology. 27:18.10:18.10.1–18.10.11.

Author Information

  1. Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire

Publication History

  1. Published Online: 1 MAR 2006
  2. Published Print: FEB 2006


This unit describes a model for the determination of host resistance in mice using a Gram-positive intracellular bacterium, Listeria monocytogenes (LM), and discusses its broad use in immunotoxicological studies. The Basic Protocol provides detailed procedures for LM infection and sample analysis, including preparation and infection of mice with LM, recording of sickness behaviors in infected mice, and determination of viable LM numbers in the tissues from infected animals. The protocol also describes use of serum and tissue homogenates for assessment of cytokines. Additionally, background information on LM and LM infection is provided for better understanding and utilization of this model. The LM infection model is useful for the initial screening of possible modulation of immune responses, particularly innate immunity and type-1 cell-mediated immunity (CMI), by environmental factors, as well as for further investigation of the underlying mechanisms of environmental factor–induced changes in immunity.


  • Listeria monocytogenes (LM);
  • Intracellular pathogen;
  • Host resistance