Unit

UNIT 20.5 CFU-MK Assay for Acute Thrombocytopenia

  1. Dominique Parent-Massin,
  2. Yann Sibiril

Published Online: 1 AUG 2008

DOI: 10.1002/0471140856.tx2005s37

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Parent-Massin, D. and Sibiril, Y. 2008. CFU-MK Assay for Acute Thrombocytopenia. Current Protocols in Toxicology. 37:20.5:20.5.1–20.5.14.

Author Information

  1. UFR Sciences, Université de Bretagne Occidentale, Brest, France

Publication History

  1. Published Online: 1 AUG 2008
  2. Published Print: AUG 2008

Abstract

In this unit, protocols to culture human platelet progenitors (Colony Forming Unit–Megakaryocyte; CFU-M) with or without toxicants are described. Platelet progenitors are obtained from human umbilical cord blood. After separation of mononuclear cells, the cell suspension can be cryopreserved or plated immediately. Megakaryocytes are identified by immunocytochemistry. Test chemicals are added to the culture medium before cells are plated. Megakaryocytes are scored after 12 days of culture. IC10, IC50 and IC90 can be calculated by comparison to control cultures. A predictive model is proposed to evaluate the hazard of thrombocytopenia induced by chemicals. When IC50 and IC90 are below Cmax in humans, the likelihood of thrombocytopenia is strong. Curr. Protoc. Toxicol. 37:20.5.1-20.5.14. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • clonogenic assays;
  • thrombocytopenia;
  • human CFU-MK;
  • predictive model