UNIT 21.4 Assessment of Ischemic Vascular Damage
Published Online: 1 MAY 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Nosál'ová, V. and Sotníková, R. 2010. Assessment of Ischemic Vascular Damage. Current Protocols in Toxicology. 44:21.4:21.4.1–21.4.21.
- Published Online: 1 MAY 2010
- Published Print: MAY 2010
This protocol describes a model convenient for acute experiments in anesthetized rats, performed by selective occlusion of the superior mesenteric artery. Such a model provides a means for assessing the role played by various pathophysiological mechanisms in the development of intestinal ischemic injury. It is especially suitable for studying different treatments, mainly pharmacological ones, to help cope with this problem in clinical practice. In the form of support protocols, this unit includes functional vascular and chemiluminescence studies, determination of vascular permeability and myeloperoxidase activity, transit time, and mortality. Curr. Protoc. Toxicol. 44:21.4.1-21.4.21. © 2010 by John Wiley & Sons, Inc.
- reactive oxygen species;
- superior mesenteric artery;