Unit

UNIT 21.4 Assessment of Ischemic Vascular Damage

  1. Viera Nosál'ová,
  2. Ruzena Sotníková

Published Online: 1 MAY 2010

DOI: 10.1002/0471140856.tx2104s44

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Nosál'ová, V. and Sotníková, R. 2010. Assessment of Ischemic Vascular Damage. Current Protocols in Toxicology. 44:21.4:21.4.1–21.4.21.

Author Information

  1. Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic

Publication History

  1. Published Online: 1 MAY 2010
  2. Published Print: MAY 2010

Abstract

This protocol describes a model convenient for acute experiments in anesthetized rats, performed by selective occlusion of the superior mesenteric artery. Such a model provides a means for assessing the role played by various pathophysiological mechanisms in the development of intestinal ischemic injury. It is especially suitable for studying different treatments, mainly pharmacological ones, to help cope with this problem in clinical practice. In the form of support protocols, this unit includes functional vascular and chemiluminescence studies, determination of vascular permeability and myeloperoxidase activity, transit time, and mortality. Curr. Protoc. Toxicol. 44:21.4.1-21.4.21. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • ischemia;
  • reperfusion;
  • reactive oxygen species;
  • rat;
  • superior mesenteric artery;
  • ileum