Unit

UNIT 21.5 Measurement of Gastric Acid Secretion in the Anaesthetized Rat

  1. Maristella Adami,
  2. Gabriella Coruzzi

Published Online: 1 AUG 2010

DOI: 10.1002/0471140856.tx2105s45

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Adami, M. and Coruzzi, G. 2010. Measurement of Gastric Acid Secretion in the Anaesthetized Rat. Current Protocols in Toxicology. 45:21.5:21.5.1–21.5.12.

Author Information

  1. University of Parma, Parma, Italy

Publication History

  1. Published Online: 1 AUG 2010
  2. Published Print: AUG 2010

Abstract

The protocols described in this unit are designed to assess the effects of substances on gastric acid secretion by the rat stomach, with the animal under general anesthesia. Both stimulatory and inhibitory effects of compounds can be evaluated and specific mechanisms of action can also be investigated. Acid secretion is induced by substances that directly activate parietal cell receptors (histamine and bethanechol), by indirect stimuli, like 2-deoxy-d-glucose, by electrical stimulation of vagal nerves, or by the peptide pentagastrin. Reference antisecretory drugs are represented by histamine H2 receptor antagonists and proton pump inhibitors. This model allows the evaluation of complete dose-response curves together with a time-course of the secretory/antisecretory effects. Indirect effects involving activation or inhibition of vagal pathways are evaluated in intact animals by means of electrical vagal stimulation or in vagotomized animals. Curr. Protoc. Toxicol. 45:21.5.1-21.5.12. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • stomach;
  • gastric acid secretion;
  • anaesthetized rat;
  • histamine;
  • pentagastrin;
  • bethanechol;
  • 2-deoxy-d-glucose;
  • vagal pathways