Unit

UNIT 21.9 Assessment of Gastrointestinal Propulsive Activity Using Three Different Models of Peristalsis In Vivo in the Mouse

  1. Enzo Poli,
  2. Cristina Pozzoli

Published Online: 1 NOV 2010

DOI: 10.1002/0471140856.tx2109s46

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Poli, E. and Pozzoli, C. 2010. Assessment of Gastrointestinal Propulsive Activity Using Three Different Models of Peristalsis In Vivo in the Mouse. Current Protocols in Toxicology. 46:21.9:21.9.1–21.9.20.

Author Information

  1. University of Parma Medical School, Parma, Italy

Publication History

  1. Published Online: 1 NOV 2010
  2. Published Print: NOV 2010

Abstract

The protocols described in this unit are designed to assess the acute effects of drugs on the propulsive activity of the gastrointestinal muscles in the conscious mouse. These protocols are currently applied to investigate the pharmacological activity of novel compounds undergoing preclinical development and to obtain predictive data needed to advance drugs into clinical trials. Moreover, these methods could be useful in evaluating the functional toxicity by environmental or alimentary pollutants, like xenobiotics and naturally occurring toxins endowed with noxious activity in the control of physiologic peristalsis. The three models detailed—the measurement of gastric emptying, ileal transit, and colonic propulsion—are substantially non-invasive and do not require analgesic pretreatments or the induction of general anesthesia. In contrast to an in vitro approach, these in vivo studies provide a unified understanding of drug effects on gut functionality, in particular when the central nervous system, the extrinsic nerves, or the (neuro)endocrine system is targeted by the test drugs.Curr. Protoc. Toxicol. 46:21.9.1-21.9.20. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • gastrointestinal tract;
  • peristaltic activity;
  • gastric emptying;
  • intestinal transit;
  • colonic transit;
  • gut propulsion