Unit

UNIT 21.12 In Vivo Measurement of Intragastric Pressure with a Rubber Balloon in the Anesthetized Rat

  1. Zoltán S. Zádori,
  2. Klára Gyires

Published Online: 23 SEP 2013

DOI: 10.1002/0471140856.tx2112s57

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Zádori, Z. S. and Gyires, K. 2013. In Vivo Measurement of Intragastric Pressure with a Rubber Balloon in the Anesthetized Rat. Current Protocols in Toxicology. 57:21.12:21.12.1–6.17.11.

Author Information

  1. Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Publication History

  1. Published Online: 23 SEP 2013

Abstract

The protocols described in this unit are designed to measure the intragastric pressure in anesthetized rats by a water-filled low-compliance rubber balloon. The balloon is introduced into the stomach either orally (by passing the balloon down the esophagus) or directly via a small incision of the fundus after laparotomy. The effects of both stimulatory (e.g., carbachol) and inhibitory (e.g., oxymetazoline) agents can be evaluated on the gastric tone and phasic contractions. The model allows the evaluation of dose-response curves and also the time-course of the effects. Furthermore, by combining centrally or peripherally acting agents the site of action can also be determined. Curr. Protoc. Toxicol. 57:21.12.1-21.12.11. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • stomach;
  • intragastric pressure;
  • rubber balloon;
  • anesthetized rat