Appendix

APPENDIX 3B Techniques for Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 AUG 2007

DOI: 10.1002/0471140856.txa03bs33

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Phelan, M. C. 2007. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Toxicology. 33:3B:A.3B.1–A.3B.18.

Author Information

  1. Molecular Pathology Laboratory Network, Maryville, Tennessee

Publication History

  1. Published Online: 1 AUG 2007
  2. Published Print: AUG 2007

This is not the most recent version of the article. View current version (1 NOV 2016)

Abstract

This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport. Curr. Protoc. Toxicol. 33:A.3B.1-A.3B.18. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • mammalian cells;
  • cell culture;
  • trypsinizing;
  • passaging;
  • hemacytometer