APPENDIX 3B Techniques for Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 AUG 2007

DOI: 10.1002/0471140856.txa03bs33

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Phelan, M. C. 2007. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Toxicology. 33:3B:A.3B.1–A.3B.18.

Author Information

  1. Molecular Pathology Laboratory Network, Maryville, Tennessee

Publication History

  1. Published Online: 1 AUG 2007
  2. Published Print: AUG 2007

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This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport. Curr. Protoc. Toxicol. 33:A.3B.1-A.3B.18. © 2007 by John Wiley & Sons, Inc.


  • mammalian cells;
  • cell culture;
  • trypsinizing;
  • passaging;
  • hemacytometer