Appendix

APPENDIX 3C Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization

  1. Martha F. Kramer,
  2. Donald M. Coen

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.txa03cs03

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Kramer, M. F. and Coen, D. M. 2001. Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization. Current Protocols in Toxicology. 3:3C:A.3C.1–A.3C.14.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000

Abstract

The polymerase chain reaction (PCR) is used to enzymatically amplify small quantities of specific DNA sequences. The reaction must optimized to specifically amplify the sequences and primers of interest; this includes titration of magnesium chloride and selection of enhancing agents, if appropriate, to minimize nonspecific primer target interactions and maximize the specificity, sensitivity, and yield.