Appendix

APPENDIX 3D Detection and Quantitation of Radiolabeled Proteins in Gels and Blots

  1. Daniel Voytas,
  2. Ning Ke

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.txa03ds07

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Voytas, D. and Ke, N. 2001. Detection and Quantitation of Radiolabeled Proteins in Gels and Blots. Current Protocols in Toxicology. 7:3D:A.3D.1–A.3D.10.

Author Information

  1. Iowa State University, Ames, Iowa

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2001

Abstract

This unit provides procedures for detecting an quantifying radiolabeled proteins in SDS-polyacrylamide gels or blots. Autoradiography requires fixed, dried gels before exposure to X-ray film. The results may be enhanced by using intensifying screens and/or preflashing the film. Alternatively fluorography with organic scintillant-impregnated gels may be used to enhance signal. The autoradiogram may be quantified by densitometry. Phosphorimaging is a useful alternative to film exposure.