Appendix

APPENDIX 3I The Colorimetric Detection and Quantitation of Total Protein

  1. Randall I. Krohn

Published Online: 1 MAR 2005

DOI: 10.1002/0471140856.txa03is23

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Krohn, R. I. 2005. The Colorimetric Detection and Quantitation of Total Protein. Current Protocols in Toxicology. 23:3I:A.3I.1–A.3I.28.

Author Information

  1. Pierce Biotechnology, Inc., Rockford, Illinois

Publication History

  1. Published Online: 1 MAR 2005
  2. Published Print: FEB 2005

Abstract

Protein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separation. Colorimetric methods are fast, simple, and not laborious. This unit describes a number of assays able to detect protein concentrations in the low microgram to milligram per milliliter ranges in a variety of formats.