UNIT 1.1 Overview of Protein Purification and Characterization

  1. R.K. Scopes

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0101s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Scopes, R. 2001. Overview of Protein Purification and Characterization. Current Protocols in Protein Science. 00:1.1:1.1.1–1.1.6.

Author Information

  1. La Trobe University, Bundoora, Australia

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995


This insightful overview by one of the most respected names in protein research discusses a broad array of the aspects involved with protein purification, including historical background, determining the purpose for purifying a particular protein, and actual methods and recommendations for purification procedures. Also covered are methods of analysis and detection, and techniques for characterizing a purified protein. Finally, a useful discussion of the equipment needed in a protein purification lab is provided.