Unit

UNIT 1.2 Strategies for Protein Purification

  1. R.K. Scopes

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0102s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Scopes, R. 2001. Strategies for Protein Purification. Current Protocols in Protein Science. 00:1.2:1.2.1–1.2.4.

Author Information

  1. La Trobe University, Bundoora, Australia

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995

Abstract

This discussion unit presents information on classification of proteins according to shape, oligomeric structure, and function. In addition, strategies for purifying different types of proteins are discussed based on their location and associations within the cell. Specifically, critical issues are detailed for extracellular, cytoplasmic, and membrane-associated proteins, as well as for insoluble proteins. Finally, strategies for the purification of both soluble and insoluble (inclusion bodies) recombinantly expressed proteins are provided.