UNIT 1.3 Protein Purification Flow Charts

  1. R.K. Scopes

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0103s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Scopes, R. 2001. Protein Purification Flow Charts. Current Protocols in Protein Science. 00:1.3:1.3.1–1.3.7.

Author Information

  1. La Trobe University, Bundoora, Australia

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995


Protein purification flow charts are presented to give a broad outline of the methods used for different types of proteins. They cannot give any detail, as the process appropriate for each protein will have its own variations at each stage. In most cases, the first stage is to obtain a solution containing the desired protein, after which it can be fractionated by various separation techniques. This unit provides charts tailored for purification of soluble and insoluble recombinant and non-recombinant proteins, as well as membrane-associated proteins.