Unit

UNIT 2.5 Sequence Similarity Searching Using the BLAST Family of Programs

  1. Tyra G. Wolfsberg,
  2. Thomas L. Madden

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0205s15

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Wolfsberg, T. G. and Madden, T. L. 2001. Sequence Similarity Searching Using the BLAST Family of Programs. Current Protocols in Protein Science. 15:2.5:2.5.1–2.5.29.

Author Information

  1. National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1999

Abstract

The BLAST (Basic Local Alignment Search Tool) family of sequence similarity search programs allows users to input either a nucleotide or amino acid query sequence, and search a nucleotide or amino acid sequence database. The program returns a list of the sequence “hits”, alignments to the query sequence, and statistical values. This unit describes how to choose an appropriate BLAST program and database, perform the search, and interpret the results.