UNIT 2.5 Sequence Similarity Searching Using the BLAST Family of Programs
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Wolfsberg, T. G. and Madden, T. L. 2001. Sequence Similarity Searching Using the BLAST Family of Programs. Current Protocols in Protein Science. 15:2.5:2.5.1–2.5.29.
- Published Online: 1 MAY 2001
- Published Print: MAR 1999
The BLAST (Basic Local Alignment Search Tool) family of sequence similarity search programs allows users to input either a nucleotide or amino acid query sequence, and search a nucleotide or amino acid sequence database. The program returns a list of the sequence “hits”, alignments to the query sequence, and statistical values. This unit describes how to choose an appropriate BLAST program and database, perform the search, and interpret the results.