UNIT 2.10 Protein Charge Determination
Published Online: 1 SEP 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Winzor, D. J. 2005. Protein Charge Determination. Current Protocols in Protein Science. 41:2.10:2.10.1–2.10.8.
- Published Online: 1 SEP 2005
- Published Print: AUG 2005
The most popular current method of determining protein valence entails the calculation of net charge from amino acid sequence/composition. However, the inaccuracy of that approach was recognized long before the advent of the protein data banks and computer programs to facilitate its adoption. Capillary zone electrophoresis affords the simplest and most economical procedure for obtaining a reliable estimate of the net charge of a protein in the buffer system of interest. This unit explains the major pitfalls in the calculation of net charge from protein sequence data.