Unit

UNIT 2.10 Protein Charge Determination

  1. Donald J. Winzor

Published Online: 1 SEP 2005

DOI: 10.1002/0471140864.ps0210s41

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Winzor, D. J. 2005. Protein Charge Determination. Current Protocols in Protein Science. 41:2.10:2.10.1–2.10.8.

Author Information

  1. University of Queensland, Brisbane, Queensland

Publication History

  1. Published Online: 1 SEP 2005
  2. Published Print: AUG 2005

Abstract

The most popular current method of determining protein valence entails the calculation of net charge from amino acid sequence/composition. However, the inaccuracy of that approach was recognized long before the advent of the protein data banks and computer programs to facilitate its adoption. Capillary zone electrophoresis affords the simplest and most economical procedure for obtaining a reliable estimate of the net charge of a protein in the buffer system of interest. This unit explains the major pitfalls in the calculation of net charge from protein sequence data.