Unit

UNIT 2.15 Bioinformatics Protocols in Glycomics and Glycoproteomics

  1. Haixu Tang1,
  2. Anoop Mayampurath1,
  3. Chuan-Yih Yu1,
  4. Yehia Mechref2

Published Online: 1 APR 2014

DOI: 10.1002/0471140864.ps0215s76

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Tang, H., Mayampurath, A., Yu, C.-Y. and Mechref, Y. 2014. Bioinformatics Protocols in Glycomics and Glycoproteomics. Current Protocols in Protein Science. 76:2.15:2.15.1–2.15.7.

Author Information

  1. 1

    School of Informatics and Computing, Indiana University, Bloomington, Indiana

  2. 2

    Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas

Publication History

  1. Published Online: 1 APR 2014

Abstract

Glycomics aims to identify the whole set of functional glycans of glycoconjugates (attached to proteins or lipids) in biological samples. Glycoproteomics aims to characterize the complete structure of all glycoproteins in biological samples, including the glycosylation sites of proteins and the various glycan structures attached to each of these sites. Mass spectrometry (MS) and microarray are high-throughput technologies that are commonly used in glycomics and glycoproteomics, which often result in the generation of large experimental datasets. Bioinformatics approaches play an essential role in automated analysis and interpretation of such data. This unit describes and discusses the computational tools currently available for these analyses, and their glycomics and glycoproteomics applications. Curr. Protoc. Protein Sci. 76:2.15.1-2.15.7. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • glycosylation;
  • glycomics;
  • glycoproteomics;
  • bioinformatics;
  • mass spectrometry;
  • microarray