UNIT 3.4 Assays for Determination of Protein Concentration
Published Online: 1 MAY 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Olson, B. J. and Markwell, J. 2007. Assays for Determination of Protein Concentration. Current Protocols in Protein Science. 48:3.4.1–3.4.29.
- Published Online: 1 MAY 2007
- Published Print: MAY 2007
Biochemical analysis of proteins relies on accurate quantitation of protein concentration. This unit describes how to perform commonly used protein assays, e.g., Lowry, Bradford, BCA, and UV spectroscopic protein assays. The primary focus of the unit is assay selection, emphasizing sample and buffer compatibility. Protein assay standard curves and data processing fundamentals are discussed in detail. This unit also details high-throughput adaptations of the commonly used protein assays, and also contains a protocol for BCA assay of total protein in SDS-PAGE sample buffer that is used for equal loading of SDS-PAGE gels, which is reliable, inexpensive, and quick.
- protein assay;