UNIT 3.5 Kinetic Assay Methods
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Allison, R. D. 2001. Kinetic Assay Methods. Current Protocols in Protein Science. 5:3.5.1–3.5.11.
- Published Online: 1 MAY 2001
- Published Print: SEP 1996
The purpose of this unit is to provide a brief review of issues important in the design of initial-rate assay methods. General aspects of kinetic assay design are discussed, including enzyme and substrate purity, concentration and stability. Also covered are issues such as continuous versus stop-time assay formats, coupled enzyme assays, binding studies, and presentation of initial-rate data.