UNIT 3.7 Metabolic Labeling with Amino Acids

  1. Juan S. Bonifacino

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0307s17

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Bonifacino, J. S. 2001. Metabolic Labeling with Amino Acids. Current Protocols in Protein Science. 17:3.7:3.7.1–3.7.10.

Author Information

  1. National Institute of Child Health and Human Development, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1999


Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This unit focuses on pulse-labeling and pulse-chase experiments done with [35S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds.