UNIT 3.7 Metabolic Labeling with Amino Acids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Bonifacino, J. S. 2001. Metabolic Labeling with Amino Acids. Current Protocols in Protein Science. 17:3.7:3.7.1–3.7.10.
- Published Online: 1 MAY 2001
- Published Print: SEP 1999
Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This unit focuses on pulse-labeling and pulse-chase experiments done with [35S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds.