UNIT 4.5 Selective Precipitation of Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Lovrien, R. E. and Matulis, D. 2001. Selective Precipitation of Proteins. Current Protocols in Protein Science. 7:4.5:4.5.1–4.5.36.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
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Selective precipitation of proteins can be used as a bulk method to recover the majority of proteins from a crude lysate, as a selective method to fractionate a subset of proteins from a protein solution, or as a very specific method to recover a single protein of interest from a purification step. This unit describes a number of methods suitable for selective precipitation. In each of the protocols that are outlined, the physical or chemical basis of the precipitation process, the parameters that can be varied for optimization, and the basic steps for developing an optimized precipitation are described.