Unit

UNIT 4.5 Selective Precipitation of Proteins

  1. Rex E. Lovrien,
  2. Daumantas Matulis

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0405s07

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Lovrien, R. E. and Matulis, D. 2001. Selective Precipitation of Proteins. Current Protocols in Protein Science. 7:4.5:4.5.1–4.5.36.

Author Information

  1. University of Minnesota, St. Paul, Minnesota

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

Selective precipitation of proteins can be used as a bulk method to recover the majority of proteins from a crude lysate, as a selective method to fractionate a subset of proteins from a protein solution, or as a very specific method to recover a single protein of interest from a purification step. This unit describes a number of methods suitable for selective precipitation. In each of the protocols that are outlined, the physical or chemical basis of the precipitation process, the parameters that can be varied for optimization, and the basic steps for developing an optimized precipitation are described.