UNIT 5.1 Production of Recombinant Proteins in Escherichia coli
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
LaVallie, E. R. 2001. Production of Recombinant Proteins in Escherichia coli. Current Protocols in Protein Science. 00:5.1:5.1.1–5.1.8.
- Published Online: 1 MAY 2001
- Published Print: JUN 1995
E. coli is the expression system of choice and a substantial body of literature has accumulated on the successful expression of foreign genes in this host. Several problems with protein expression in E. coli have been encountered, and many have been ultimately solved. This unit describes methods that have been developed for production of recombinant proteins in E. coli and potential pitfalls that may be encountered.