Unit

UNIT 5.1 Production of Recombinant Proteins in Escherichia coli

  1. Edward R. LaVallie

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0501s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

LaVallie, E. R. 2001. Production of Recombinant Proteins in Escherichia coli. Current Protocols in Protein Science. 00:5.1:5.1.1–5.1.8.

Author Information

  1. Genetics Institute, Inc., Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995

Abstract

E. coli is the expression system of choice and a substantial body of literature has accumulated on the successful expression of foreign genes in this host. Several problems with protein expression in E. coli have been encountered, and many have been ultimately solved. This unit describes methods that have been developed for production of recombinant proteins in E. coli and potential pitfalls that may be encountered.