UNIT 5.12 Preparation of Cell Cultures and Vaccinia Virus Stocks
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Earl, P. L., Cooper, N., Wyatt, L. S., Moss, B. and Carroll, M. W. 2001. Preparation of Cell Cultures and Vaccinia Virus Stocks. Current Protocols in Protein Science. 13:5.12:5.12.1–5.12.12.
- Published Online: 1 MAY 2001
- Published Print: SEP 1998
This unit describes the maintenance of cell lines used with vaccinia virus, both in monolayer cultures and in suspension. The suspended cell culture is then used in the preparation of vaccinia virus stocks. The preparation of chick embryo fibroblasts (CEF) is also presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Additionally, support protocols are presented for the titration of standard and MVA vaccinia virus stocks.