Unit

UNIT 5.13 Generation of Recombinant Vaccinia Viruses

  1. Patricia L. Earl1,
  2. Bernard Moss1,
  3. Linda S. Wyatt1,
  4. Miles W. Carroll2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0513s13

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Earl, P. L., Moss, B., Wyatt, L. S. and Carroll, M. W. 2001. Generation of Recombinant Vaccinia Viruses. Current Protocols in Protein Science. 13:5.13:5.13.1–5.13.19.

Author Information

  1. 1

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

  2. 2

    Oxford BioMedica, Oxford, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998

Abstract

This unit first describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector to generate a recombinant virus. Methods are also presented for purifying vaccinia virus and for isolating viral DNA, which can be used during transfection. Also presented are selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented.