UNIT 5.16 Choice of Cellular Protein Expression System
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Gray, D. and Subramanian, S. 2001. Choice of Cellular Protein Expression System. Current Protocols in Protein Science. 20:5.16:5.16.1–5.16.34.
- Published Online: 1 MAY 2001
- Published Print: JUN 2000
Recombinant protein expression has become a standard laboratory tool, and a wide variety of systems and techniques are now in use. Because there are so many systems to choose from, the investigator has to be careful to use the combination that will give the best results for the protein being studied. This overview unit discusses expression and production choices, including post-translational modifications (e.g., glycosylation, acylation, sulfation, and removal of N-terminal methionine), in vivo and in vitro folding, and influence of downstream elements on expression.