Unit

UNIT 5.16 Choice of Cellular Protein Expression System

  1. David Gray1,
  2. Shyam Subramanian2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0516s20

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Gray, D. and Subramanian, S. 2001. Choice of Cellular Protein Expression System. Current Protocols in Protein Science. 20:5.16:5.16.1–5.16.34.

Author Information

  1. 1

    Chiron Corporation, Emeryville, California

  2. 2

    Merck Research Laboratories, West Point, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 2000

Abstract

Recombinant protein expression has become a standard laboratory tool, and a wide variety of systems and techniques are now in use. Because there are so many systems to choose from, the investigator has to be careful to use the combination that will give the best results for the protein being studied. This overview unit discusses expression and production choices, including post-translational modifications (e.g., glycosylation, acylation, sulfation, and removal of N-terminal methionine), in vivo and in vitro folding, and influence of downstream elements on expression.