Unit

UNIT 5.23 Autoinduction of Protein Expression

  1. Brian G. Fox,
  2. Paul G. Blommel

Published Online: 1 APR 2009

DOI: 10.1002/0471140864.ps0523s56

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Fox, B. G. and Blommel, P. G. 2009. Autoinduction of Protein Expression. Current Protocols in Protein Science. 56:5.23:5.23.1–5.23.18.

Author Information

  1. Department of Biochemistry, Biophysics Degree Program, and Center for Eukaryotic Structural Genomics, University of Wisconsin–Madison, Madison, Wisconsin

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009

Abstract

This unit contains protocols for the use of lactose-derived autoinduction in Escherichia coli. The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included. Curr. Protoc. Protein Sci. 56:5.23.1-5.23.18. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • recombinant protein;
  • expression;
  • Escherichia coli;
  • high-throughput methods;
  • X-ray crystallography