UNIT 5.23 Autoinduction of Protein Expression
Published Online: 1 APR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Fox, B. G. and Blommel, P. G. 2009. Autoinduction of Protein Expression. Current Protocols in Protein Science. 56:5.23:5.23.1–5.23.18.
- Published Online: 1 APR 2009
- Published Print: APR 2009
This unit contains protocols for the use of lactose-derived autoinduction in Escherichia coli. The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included. Curr. Protoc. Protein Sci. 56:5.23.1-5.23.18. © 2009 by John Wiley & Sons, Inc.
- recombinant protein;
- Escherichia coli;
- high-throughput methods;
- X-ray crystallography