Unit

UNIT 5.25 Overview of Approaches to Preventing and Avoiding Proteolysis During Expression and Purification of Proteins

  1. Barry J. Ryan,
  2. Gary T. Henehan

Published Online: 1 FEB 2013

DOI: 10.1002/0471140864.ps0525s71

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Ryan, B. J. and Henehan, G. T. 2013. Overview of Approaches to Preventing and Avoiding Proteolysis During Expression and Purification of Proteins. Current Protocols in Protein Science. 71:5.25:5.25.1–5.25.7.

Author Information

  1. Food Science and Environmental Health, Dublin Institute of Technology, Dublin, Ireland

Publication History

  1. Published Online: 1 FEB 2013
  2. Published Print: FEB 2013

Abstract

Proteases are enzymes that cleave proteins. They occur widely in nature and play a fundamental role in cellular homeostasis; however, their presence can result in unwanted protein degradation during recombinant protein expression and purification. This unit introduces proteases, specifically focusing on the types commonly encountered during production of recombinant proteins. The strategies used to avoid and to prevent proteolysis are also described, with extensive consideration of the molecular, technical, and logistic methodologies involved. Curr. Protoc. Protein Sci. 71:5.25.1-5.25.7. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • protease;
  • proteolysis;
  • recombinant protein