Unit

UNIT 6.10 E. coli Expression and Purification of Fab Antibody Fragments

  1. Ka Yin Kwong,
  2. Christoph Rader

Published Online: 1 FEB 2009

DOI: 10.1002/0471140864.ps0610s55

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Kwong, K. Y. and Rader, C. 2009. E. coli Expression and Purification of Fab Antibody Fragments. Current Protocols in Protein Science. 55:6.10:6.10.1–6.10.14.

Author Information

  1. Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2009
  2. Published Print: FEB 2009

Abstract

The Fab molecule was the first generated antibody fragment and still dominates basic research and clinical applications. This unit describes the E. coli expression and purification of Fab antibody fragments with and without a His tag, and is designed to yield sufficient protein for the evaluation and characterization of a panel of Fab selected from a Fab library by phage display. Curr. Protoc. Protein Sci. 55:6.10.1-6.10.14. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • Fab;
  • E. coli;
  • His tag;
  • IMAC;
  • affinity chromatography