UNIT 6.11 Elastin-Like Polypeptides as a Purification Tag for Recombinant Proteins
Published Online: 1 AUG 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Hassouneh, W., Christensen, T. and Chilkoti, A. 2010. Elastin-Like Polypeptides as a Purification Tag for Recombinant Proteins. Current Protocols in Protein Science. 61:6.11:6.11.1–6.11.16.
- Published Online: 1 AUG 2010
- Published Print: AUG 2010
This unit presents a recombinant protein purification method that employs an elastin-like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature. ELPs retain this behavior when they are fused to a protein, and thereby provide a simple method to isolate a recombinant ELP fusion protein from cell contaminants by cycling the solution through the insoluble and soluble phase of the ELP fusion protein using a procedure that is termed Inverse Transition Cycling. This method does not require the use of chromatography, so it is cost-effective, easy to scale up, and easy to multiplex. Curr. Protoc. Protein Sci. 61:6.11.1-6.11.16. © 2010 by John Wiley & Sons, Inc.
- Elastin-like polypeptides (ELP);
- inverse transition cycling (ITC);
- purification tag;
- recombinant protein;
- non-chromatographic purification;
- protein fusion;
- transition temperature