Unit

UNIT 7.6 Determining the CD Spectrum of a Protein

  1. Roger Pain

Published Online: 1 JAN 2005

DOI: 10.1002/0471140864.ps0706s38

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Pain, R. 2005. Determining the CD Spectrum of a Protein. Current Protocols in Protein Science. 38:7.6:7.6.1–7.6.24.

Author Information

  1. Jozef Stefan Institute, Ljubljana, Slovenia

Publication History

  1. Published Online: 1 JAN 2005
  2. Published Print: NOV 2004

Abstract

This unit describes the theory behind circular dichroism (CD) and deals with considerations regarding instrumentation and reagents for CD spectrometry. A protocol is provided that outlines the steps in recording a CD spectrum and two support protocols explain the interpretation of near-UV and far-UV CD spectra.