Unit

UNIT 7.9 Measuring Protein Thermostability by Differential Scanning Calorimetry

  1. George I. Makhatadze

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0709s12

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Makhatadze, G. I. 2001. Measuring Protein Thermostability by Differential Scanning Calorimetry. Current Protocols in Protein Science. 12:7.9:7.9.1–7.9.14.

Author Information

  1. Texas Tech University, Lubbock, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1998

Abstract

Differential scanning calorimetry (DSC) the method of choice to study the conformational stability of biological macromolecules and proteins in particular. This unit presents step-by-step protocols for DSC, including sample preparation and interpretation of the results in the simplest cases as well as calibration of the apparatus and maintenance of DSC cells. Various general experimental considerations, including possible pitfalls and errors, are also discussed.