UNIT 7.9 Measuring Protein Thermostability by Differential Scanning Calorimetry
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Makhatadze, G. I. 2001. Measuring Protein Thermostability by Differential Scanning Calorimetry. Current Protocols in Protein Science. 12:7.9:7.9.1–7.9.14.
- Published Online: 1 MAY 2001
- Published Print: MAY 1998
Differential scanning calorimetry (DSC) the method of choice to study the conformational stability of biological macromolecules and proteins in particular. This unit presents step-by-step protocols for DSC, including sample preparation and interpretation of the results in the simplest cases as well as calibration of the apparatus and maintenance of DSC cells. Various general experimental considerations, including possible pitfalls and errors, are also discussed.