Miscellaneous
UNIT 7.13 Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins
Published Online: 1 APR 2010
DOI: 10.1002/0471140864.ps0713s60
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
Additional Information
How to Cite
Demeler, B. 2010. Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins. Current Protocols in Protein Science. 60:7.13.1–7.13.24.
Publication History
- Published Online: 1 APR 2010
- Published Print: APR 2010
- Abstract
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Analytical ultracentrifugation experiments play an integral role in the solution phase characterization of recombinant proteins and other biological macromolecules. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Optima XL-A or XL-I analytical ultracentrifuge. Optimal instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments. Curr. Protoc. Protein Sci. 60:7.13.1-7.13.24. © 2010 by John Wiley & Sons, Inc.
Keywords: analytical ultracentrifugation; solution studies; sedimentation velocity; sedimentation equilibrium; UltraScan; 2-dimensional spectrum analysis; absorbance optics; intensity measurements