UNIT 8.1 Overview of Conventional Chromatography
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Williams, A. 2001. Overview of Conventional Chromatography. Current Protocols in Protein Science. 00:8.1.1–8.1.9.
- Published Online: 1 MAY 2001
- Published Print: JUN 1995
The key to success in purification of any protein is developing the right separation strategy. This involves selecting and correctly applying a combination of separation techniques, chosen on the basis of information about the target protein and contaminants. There is no single separation technique and no single purification scheme that will allow successful purification of all types of proteins. However, there is a single approach to developing a successful purification strategy. This overview discusses the basic steps that must be taken, including selection of source material, and progression from initial “capture” stage of separation to the final polishing stage. Factors that can affect chromatographic resolution are also covered.