UNIT 8.3 Gel-Filtration Chromatography

  1. Lars Hagel

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0803s14

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Hagel, L. 2001. Gel-Filtration Chromatography. Current Protocols in Protein Science. 14:8.3:8.3.1–8.3.30.

Author Information

  1. Amersham Pharmacia Biotech AB, Uppsala, Sweden

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1998


Three basic protocols are provided in this unit, corresponding to the major applications of gel filtration. One method describes desalting or group separation, in which the target protein and contaminating solutes differ substantially in molecular size. Another is used for protein fractionation, application is in characterizing the molecular dimensions of proteins. A Support Protocol is also provided for calibrating gel filtration columns to be used in estimating molecular size.