UNIT 8.4 Hydrophobic-Interaction Chromatography
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Kennedy, R. M. 2001. Hydrophobic-Interaction Chromatography. Current Protocols in Protein Science. 00:8.4:8.4.1–8.4.21.
- Published Online: 1 MAY 2001
- Published Print: JUN 1995
This unit discusses the important parameters in designing and optimizing a separation by hydrophobic interaction chromatography (HIC), including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for packing and testing a column; determining binding and elution conditions; eluting the sample; and cleaning, regenerating, and storing HIC columns.