UNIT 8.4 Hydrophobic-Interaction Chromatography

  1. Robert M. Kennedy

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0804s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Kennedy, R. M. 2001. Hydrophobic-Interaction Chromatography. Current Protocols in Protein Science. 00:8.4:8.4.1–8.4.21.

Author Information

  1. Pharmacia Biotech, Piscataway, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995


This unit discusses the important parameters in designing and optimizing a separation by hydrophobic interaction chromatography (HIC), including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for packing and testing a column; determining binding and elution conditions; eluting the sample; and cleaning, regenerating, and storing HIC columns.