UNIT 8.7 HPLC of Peptides and Proteins

  1. Reinhard I. Boysen,
  2. Milton T.W. Hearn

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0807s23

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Boysen, R. I. and Hearn, M. T. 2001. HPLC of Peptides and Proteins. Current Protocols in Protein Science. 23:8.7:8.7.1–8.7.40.

Author Information

  1. Monash University, Victoria, Australia

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 2001

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High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This is an excellent primer for HPLC users.