UNIT 8.7 HPLC of Peptides and Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Boysen, R. I. and Hearn, M. T. 2001. HPLC of Peptides and Proteins. Current Protocols in Protein Science. 23:8.7:8.7.1–8.7.40.
- Published Online: 1 MAY 2001
- Published Print: MAR 2001
This is not the most recent version of the article. View current version (1 AUG 2010)
High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This is an excellent primer for HPLC users.