UNIT 8.9 Displacement Chromatography of Proteins
Published Online: 1 FEB 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
McAtee, C. P. 2010. Displacement Chromatography of Proteins. Current Protocols in Protein Science. 59:8.9:8.9.1–8.9.14.
- Published Online: 1 FEB 2010
- Published Print: FEB 2010
This unit discusses the important parameters in designing and optimizing a separation by ion-exchange displacement chromatography, including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for testing a column, determining binding and elution conditions, displacing the sample, and cleaning, regenerating, and storing of displacement columns. Curr. Protoc. Protein Sci. 59:8.9.1-8.9.14. © 2010 by John Wiley & Sons, Inc.
- ion-exchange chromatography