UNIT 8.9 Displacement Chromatography of Proteins

  1. C. Patrick McAtee

Published Online: 1 FEB 2010

DOI: 10.1002/0471140864.ps0809s59

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

McAtee, C. P. 2010. Displacement Chromatography of Proteins. Current Protocols in Protein Science. 59:8.9:8.9.1–8.9.14.

Author Information

  1. SACHEM, Inc., Austin, Texas

Publication History

  1. Published Online: 1 FEB 2010
  2. Published Print: FEB 2010


This unit discusses the important parameters in designing and optimizing a separation by ion-exchange displacement chromatography, including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for testing a column, determining binding and elution conditions, displacing the sample, and cleaning, regenerating, and storing of displacement columns. Curr. Protoc. Protein Sci. 59:8.9.1-8.9.14. © 2010 by John Wiley & Sons, Inc.


  • displacement;
  • HPLC;
  • ion-exchange chromatography