UNIT 8.10 Isolation of Monoclonal Antibody Charge Variants by Displacement Chromatography
Published Online: 1 AUG 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
McAtee, C. P. and Hornbuckle, J. 2012. Isolation of Monoclonal Antibody Charge Variants by Displacement Chromatography. Current Protocols in Protein Science. 69:8.10:8.10.1–8.10.13.
- Published Online: 1 AUG 2012
- Published Print: AUG 2012
This unit discusses the important parameters in designing and optimizing a separation of monoclonal antibody (mAb) charge variants from process streams by ion-exchange displacement chromatography, including sample preparation and selection of matrix, column, and appropriate buffer. A protocol is provided for determination of optimal column binding and displacement conditions, including cleaning and regeneration of the displacement columns. Curr. Protoc. Protein Sci. 69:8.10.1-8.10.13. © 2012 by John Wiley & Sons, Inc.
- displacement chromatography;
- ion-exchange chromatography;
- monoclonal antibodies;
- charge variants;
- quality by design