Unit

UNIT 9.1 Lectin Affinity Chromatography

  1. Hudson H. Freeze

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps0901s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Freeze, H. H. 2001. Lectin Affinity Chromatography. Current Protocols in Protein Science. 00:9.1:9.1.1–9.1.9.

Author Information

  1. La Jolla Cancer Research Foundation, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995

Abstract

This unit describes the use of lectins for preparative glycoprotein purification. Con A-Sepharose and WGA-agarose are used for convenience and availability. Instructions are given for a small-scale pilot procedure to test for lectin binding and to determine elution conditions. There are many variations on the basic procedure in the literature, but all use the same principles: bind the protein to immobilized lectin through its sugar chain, wash away unbound protein, and elute bound protein with a simple sugar that resembles the sugar ligand of the bound protein.