Unit

UNIT 9.9 Overview of Affinity Tags for Protein Purification

  1. Michelle E. Kimple1,
  2. John Sondek2

Published Online: 1 SEP 2004

DOI: 10.1002/0471140864.ps0909s36

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Kimple, M. E. and Sondek, J. 2004. Overview of Affinity Tags for Protein Purification. Current Protocols in Protein Science. 36:9.9:9.9.1–9.9.19.

Author Information

  1. 1

    Duke University Medical Center, Durham, North Carolina

  2. 2

    University of North Carolina Chapel Hill, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 SEP 2004
  2. Published Print: MAY 2004

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Abstract

Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, and for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.

Keywords:

  • protein purification;
  • protein detection;
  • affinity chromatography;
  • affinity tag;
  • epitope tag
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