UNIT 9.9 Overview of Affinity Tags for Protein Purification

  1. Michelle E. Kimple,
  2. Allison L. Brill,
  3. Renee L. Pasker

Published Online: 24 SEP 2013

DOI: 10.1002/0471140864.ps0909s73

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Kimple, M. E., Brill, A. L. and Pasker, R. L. 2013. Overview of Affinity Tags for Protein Purification. Current Protocols in Protein Science. 73:9.9:9.9.1–9.9.23.

Author Information

  1. University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin

Publication History

  1. Published Online: 24 SEP 2013


Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, as well as for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future. Curr. Protoc. Protein Sci. 73:9.9.1-9.9.23. © 2013 by John Wiley & Sons, Inc.


  • protein purification;
  • protein detection;
  • affinity chromatography;
  • affinity tag;
  • epitope tag