UNIT 10.3 One-Dimensional Electrophoresis Using Nondenaturing Conditions

  1. Sean R. Gallagher

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1003s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Gallagher, S. R. 2001. One-Dimensional Electrophoresis Using Nondenaturing Conditions. Current Protocols in Protein Science. 00:10.3:10.3.1–10.3.11.

Author Information

  1. Hoefer Pharmacia Biotech, San Francisco, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995


Nondenaturing or “native“ electrophoresis—i.e., electrophoresis in the absence of denaturants such as detergents and urea—is an often-overlooked technique for determining the native size, subunit structure, and optimal separation of a protein. Two protocols are presented in this unit: continuous PAGE, which is highly flexible, permitting cationic and anionic electrophoresis over a full range of pH, and discontinuous PAGE, which is limited to proteins negatively charged at neutral pH but provides high resolution for accurate size calibration.