Unit

UNIT 10.7 Electroblotting from Polyacrylamide Gels

  1. Jeanine A. Ursitti,
  2. Jacek Mozdzanowski,
  3. David W. Speicher

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1007s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Ursitti, J. A., Mozdzanowski, J. and Speicher, D. W. 2001. Electroblotting from Polyacrylamide Gels. Current Protocols in Protein Science. 00:10.7:10.7.1–10.7.14.

Author Information

  1. The Wistar Institute, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995

Abstract

This unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane. An alternate protocol is provided for electroblotting in semidry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents.