UNIT 10.7 Electroblotting from Polyacrylamide Gels
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Ursitti, J. A., Mozdzanowski, J. and Speicher, D. W. 2001. Electroblotting from Polyacrylamide Gels. Current Protocols in Protein Science. 00:10.7:10.7.1–10.7.14.
- Published Online: 1 MAY 2001
- Published Print: JUN 1995
This unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane. An alternate protocol is provided for electroblotting in semidry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents.