Unit

UNIT 10.8 Detection of Proteins on Blot Membranes

  1. Sandra Harper,
  2. David W. Speicher

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1008s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Harper, S. and Speicher, D. W. 2001. Detection of Proteins on Blot Membranes. Current Protocols in Protein Science. 00:10.8:10.8.1–10.8.7.

Author Information

  1. The Wistar Institute, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995

Abstract

Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. In the protocols described in this unit, proteins are stained after electroblotting from one-dimensional or two-dimensional polyacrylamide gels to blot membranes such as polyvinylidene difluoride (PVDF), nitrocellulose, or nylon membranes. Protocols are provided for the use of six general protein stains: amido black, Coomassie blue, Ponceau S, colloidal gold, colloidal silver, and India ink. In addition, the fluorescent stains fluorescamine and IAEDANS, which covalently react with bound proteins, are described. Approximate detection limits for each nonfluorescent stain are indicated along with membrane compatibilities.