UNIT 10.11 Autoradiography

  1. Daniel C. Bundy

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1011s10

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Bundy, D. C. 2001. Autoradiography. Current Protocols in Protein Science. 10:10.11:10.11.1–10.11.6.

Author Information

  1. Eastman Kodak Company, New Haven, Connecticut

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1997


This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope-labeled substances within a membrane or specimen material. The various types of film are discussed along with instructions on their correct handling. Quantitative autoradiography is described as a means of calibrating experimental results. Importantly, specific guidelines are given for pre-flashing film, along with exposure and processing procedures.