UNIT 10.11 Autoradiography
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Bundy, D. C. 2001. Autoradiography. Current Protocols in Protein Science. 10:10.11:10.11.1–10.11.6.
- Published Online: 1 MAY 2001
- Published Print: DEC 1997
This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope-labeled substances within a membrane or specimen material. The various types of film are discussed along with instructions on their correct handling. Quantitative autoradiography is described as a means of calibrating experimental results. Importantly, specific guidelines are given for pre-flashing film, along with exposure and processing procedures.