Unit

UNIT 10.12 Overview of Digital Electrophoresis Analysis

  1. Scott Medberry1,
  2. Sean Gallagher2,
  3. Butch Moomaw3

Published Online: 1 SEP 2005

DOI: 10.1002/0471140864.ps1012s41

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Medberry, S., Gallagher, S. and Moomaw, B. 2005. Overview of Digital Electrophoresis Analysis. Current Protocols in Protein Science. 41:10.12:10.12.1–10.12.25.

Author Information

  1. 1

    Agilent Technologies, Inc., Palo Alto, California

  2. 2

    UVP, Inc., Upland, California

  3. 3

    Hamamatsu Photonic Systems, Spring Branch, Texas

Publication History

  1. Published Online: 1 SEP 2005
  2. Published Print: AUG 2005

Abstract

Gel electrophoresis has become a ubiquitous method in molecular biology for separating biomolecules. This prominence is the result of several factors, including the robustness, speed, and potentially high throughput of the technique. The results of this method are traditionally documented using silver halide-based photography followed by manual interpretation. While this remains an excellent method for qualitative documentation of single-gel results, digital capture offers a number of significant advantages when documentation requires quantitation and sophisticated analysis. Digital images of gel electropherograms can be obtained rapidly using an image-capture device, and the images can be easily manipulated using image analysis software. This overview presents reasons for digital documentation and analysis, defines some important key terms for imaging, explains the capture process and reviews the devices used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis.

Keywords:

  • electrophoresis;
  • imaging;
  • software;
  • digital analysis;
  • quantitation