Unit

UNIT 10.13 Capillary Electrophoresis of Peptides and Proteins Using Isoelectric Buffers

  1. Pier Giorgio Righetti1,
  2. Alessandra Bossi1,
  3. Cecilia Gelfi2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1013s16

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Righetti, P. G., Bossi, A. and Gelfi, C. 2001. Capillary Electrophoresis of Peptides and Proteins Using Isoelectric Buffers. Current Protocols in Protein Science. 16:10.13:10.13.1–10.13.16.

Author Information

  1. 1

    University of Verona, Verona, Italy

  2. 2

    Instituto Tecnologie Biomediche Avanzate and Consiglio Nazionale delle Richerce, Milan, Italy

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1999

Abstract

Capillary electrophoresis in acidic isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and no interaction of macromolecules with the untreated wall ensues. In addition, because of the low conductivity of quasi-stationary isoelectric buffers, high voltage gradients can be applied (up to 800 V/cm), permitting fast peptide analysis with a high resolving power and minimal diffusional peak spreading.