UNIT 10.13 Capillary Electrophoresis of Peptides and Proteins Using Isoelectric Buffers
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Righetti, P. G., Bossi, A. and Gelfi, C. 2001. Capillary Electrophoresis of Peptides and Proteins Using Isoelectric Buffers. Current Protocols in Protein Science. 16:10.13:10.13.1–10.13.16.
- Published Online: 1 MAY 2001
- Published Print: JUN 1999
Capillary electrophoresis in acidic isoelectric buffers is a novel methodology allowing fast protein and peptide analysis in uncoated capillaries. Due to the low pH adopted and to the use of dynamic coating with cellulose derivatives, silanol ionization is essentially suppressed and no interaction of macromolecules with the untreated wall ensues. In addition, because of the low conductivity of quasi-stationary isoelectric buffers, high voltage gradients can be applied (up to 800 V/cm), permitting fast peptide analysis with a high resolving power and minimal diffusional peak spreading.