Unit

UNIT 12.8 Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine

  1. Natasha E. Zachara1,
  2. Keith Vosseller2,
  3. Gerald W. Hart1

Published Online: 1 NOV 2011

DOI: 10.1002/0471140864.ps1208s66

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Zachara, N. E., Vosseller, K. and Hart, G. W. 2011. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine. Current Protocols in Protein Science. 66:12.8:12.8.1–12.8.33.

Author Information

  1. 1

    The Johns Hopkins University School of Medicine, Baltimore, Maryland

  2. 2

    Drexel University College of Medicine, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 NOV 2011
  2. Published Print: NOV 2011

Abstract

O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites. Curr. Protoc. Protein Sci. 66:12.8.1-12.8.33. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • signal transduction;
  • glycosylation;
  • O-GlcNAc;
  • method(s);
  • detection;
  • analysis;
  • galactosyltransferase;
  • O-linked;
  • glycosylation