Unit

UNIT 12.9 High-Throughput Lectin Microarray-Based Analysis of Live Cell Surface Glycosylation

  1. Yu Li1,
  2. Sheng-ce Tao2,3,4,
  3. Heng Zhu2,3,
  4. Jonathan P. Schneck1

Published Online: 1 FEB 2011

DOI: 10.1002/0471140864.ps1209s63

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Li, Y., Tao, S.-c., Zhu, H. and Schneck, J. P. 2011. High-Throughput Lectin Microarray-Based Analysis of Live Cell Surface Glycosylation. Current Protocols in Protein Science. 63:12.9:12.9.1–12.9.7.

Author Information

  1. 1

    Department of Pathology, Oncology & Medicine and Institute for Cell Engineering, Johns Hopkins University, Baltimore, Maryland

  2. 2

    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland

  3. 3

    The High-Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland

  4. 4

    Shanghai Center for Systems Biomedicine, Shanghai Jiaotong University, Shanghai, China

Publication History

  1. Published Online: 1 FEB 2011
  2. Published Print: FEB 2011

Abstract

Lectins, plant-derived glycan-binding proteins, have long been used to detect glycans on cell surfaces. However, the techniques used to characterize serum or cells have largely been limited to mass spectrometry, blots, flow cytometry, and immunohistochemistry. While these lectin-based approaches are well established and they can discriminate a limited number of sugar isomers by concurrently using a limited number of lectins, they are not amenable for adaptation to a high-throughput platform. Fortunately, given the commercial availability of lectins with a variety of glycan specificities, lectins can be printed on a glass substrate in a microarray format to profile accessible cell-surface glycans. This method is an inviting alternative for analysis of a broad range of glycans in a high-throughput fashion and has been demonstrated to be a feasible method of identifying binding-accessible cell surface glycosylation on living cells. The current unit presents a lectin-based microarray approach for analyzing cell surface glycosylation in a high-throughput fashion. Curr. Protoc. Protein Sci. 63:12.9.1-12.9.7. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • microarrays;
  • lectins;
  • glycosylation;
  • cancer stem cells;
  • immunology;
  • infectious diseases