UNIT 12.10 Preparation and Analysis of Glycan Microarrays
Published Online: 1 APR 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Heimburg-Molinaro, J., Song, X., Smith, D. F. and Cummings, R. D. 2011. Preparation and Analysis of Glycan Microarrays. Current Protocols in Protein Science. 64:12.10:12.10.1–12.10.29.
- Published Online: 1 APR 2011
- Published Print: APR 2011
Determination of the binding specificity of glycan-binding proteins (GBPs), such as lectins, antibodies, and receptors, has traditionally been difficult and laborious. The advent of glycan microarrays has revolutionized the field of glycobiology by allowing simultaneous screening of a GBP for interactions with a large set of glycans in a single format. This unit describes the theory and method for production of two types of glycan microarrays (chemo/enzymatically synthesized and naturally derived), and their application to functional glycomics to explore glycan recognition by GBPs. These procedures are amenable to various types of arrays and a wide range of GBP samples. Curr. Protoc. Protein Sci. 64:12.10.1-12.10.29. © 2011 by John Wiley & Sons, Inc.
- glycan microarrays;
- natural glycan microarrays;
- glycan-binding specificities;
- Consortium for Functional Glycomics